Application

Autoactivated on column by incubating with Mg/ATP, excess ATP & MgAc removed by multiple column wash steps.

Research CategoryInflammation & Immunology

Biochem/physiol Actions

Target Sub-Family: TK

Protein Target: IGF-1R

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: Expressed by baculovirus in Sf21 insect cells.

N-terminal 6His-tagged, recombinant, human IGF-1R amino acids 959-end, expressed by baculovirus in Sf21 insect cells. Autoactivated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size --call for pricing and availability and reference catalog number 14-802M when ordering the 250µg size.

Physical form

Ni²⁺/NTA-agarose

50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.

Storage and Stability

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.